tag:blogger.com,1999:blog-6282293920238017122.post1136644364175063535..comments2024-03-02T00:18:22.263-08:00Comments on The Sense Strand: Things worth mentioning about competence genes in competence mutantsRosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-6282293920238017122.post-86058596413745718292015-06-09T19:30:13.677-07:002015-06-09T19:30:13.677-07:00Wow - sxy1 and rpoD are strikingly similar, even w...Wow - sxy1 and rpoD are strikingly similar, even without the only 1 replicate for rpoD. Do they have similar hyper competence in the time courses? The work we did with the beta-gal fusions suggested that rpoD upregulated sxy transcription. we didn't see evidence of increased translation but I don't think we could rule it out either. I have to look again to see how sxy transcript levels at in rpoD753 compare to sxy transcript levels in KW20 and also to sxy transcript levels in sxy-1. Laurihttps://www.blogger.com/profile/05584157319278511653noreply@blogger.comtag:blogger.com,1999:blog-6282293920238017122.post-62088831209427567172015-06-02T08:02:35.695-07:002015-06-02T08:02:35.695-07:00About hsdR. It might be relevant to DNA uptake, s...About hsdR. It might be relevant to DNA uptake, since it includes a DNA-translocation motor that can pull thousands of bp of DNA (see http://genesdev.cshlp.org/content/26/1/92.short).<br /><br />This paper also says: "...mobile genetic elements, including phage and conjugative transposons and plasmids, have developed an extensive range of anti-restriction measures<br />to overcome the RM barrier". Toxin-antitoxin systems are also thought to be protective measures evolved by transposons and plasmids, so maybe the toxin's real job is blocking translocation by the hsdR system, and a side effect is that it blocks DNA uptake! <br /><br />That might explain why the antitoxin knockout completely blocks DNA uptake and transformation but only modestly reduces expression of competence genes.<br /><br />Is hsdR expression only up in the double (toxin-antitoxin) knockout, not in the toxin knockout? Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-6282293920238017122.post-15609062083669716242015-06-02T07:39:52.319-07:002015-06-02T07:39:52.319-07:00I'm just starting to go through all this. I&#...I'm just starting to go through all this. I'm interested in the few cases where gene expression goes down below the KW20-M0 baseline, but the graphs appear to be cut off at about a 2-fold decrease.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.com